Amulet Of Horus
The amulet is needed to activate the Armour of Horus, which Lara had recovered. Upon her arrival to the Temple of Horus, Lara placed the amulet on the armour, which shortly was destroyed by Seth, knocking the amulet into the surrounding water. Lara recovered the amulet, avoided Seth's attacks and climbed out of the temple. She placed the amulet on a trapdoor, closing it and sealing Seth within the temple for all time. Lara cannot retrieve the amulet since it would just unleash Seth again.
The amulet is actually an Ankh with hieroglyphic signs denoting life.
Powers
The amulet had the power to seal Seth in his tomb so long as it remained on his sarcophagus. This was undermined when Lara removed it. The amulet also has the power to close a trapdoor within the Temple of Horus, which Lara used to seal Seth within.
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